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https://locus.ufv.br//handle/123456789/18386
Tipo: | Artigo |
Título: | Differential cellular immune response of Galleria mellonella to Actinobacillus pleuropneumoniae |
Autor(es): | Blanco, Luis Andrés Arteaga Crispim, Josicelli Souza Fernandes, Kenner Morais Oliveira, Leandro Licursi de Pereira, Monalessa Fábia Bazzolli, Denise Mara Soares Martins, Gustavo Ferreira |
Abstract: | In the present work, we have investigate the cellular immune response of Galleria mellonella larvae against three strains of the gram-negative bacterium Actinobacillus pleuropneumoniae: low-virulence (780), high-virulence (1022) and the serotype 8 reference strain (R8). Prohemocytes, plasmatocytes, granulocytes, oenocytoids and spherulocytes were distinguished according to their size and morphology, their molecular markers and dye-staining properties and their role in the immune response. Total hemocyte count, differential hemocyte count, lysosome activity, autophagic response, cell viability and caspase-3 activation were determined in circulating hemocytes of naive and infected larvae. The presence of the autophagosome protein LC3 A/B within the circulating hemocytes of G. mellonella was dependent on and related to the infecting A. pleuropneumoniae strain and duration of infection. Hemocytes treated with the high-virulence strain expressed higher levels of LC3 A/B, whereas treatment with the low-virulence strain induced lower expression levels of this protein in the cells. Moreover, our results showed that apoptosis in circulating hemocytes of G. mellonella larvae after exposure to virulent bacterial strains occurred simultaneously with excessive cell death response induced by stress and subsequent caspase-3 activation. |
Palavras-chave: | Apoptosis Autophagy Immune response Actinobacillus pleuropneumoniae Galleria mellonella |
Editor: | Cell and Tissue Research |
Tipo de Acesso: | Springer Berlin Heidelberg |
URI: | https://doi.org/10.1007/s00441-017-2653-5 http://www.locus.ufv.br/handle/123456789/18386 |
Data do documento: | 8-Jul-2017 |
Aparece nas coleções: | Artigos |
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Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 7,79 MB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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