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https://locus.ufv.br//handle/123456789/18171
Tipo: | Artigo |
Título: | Efficient and new method for Tectona grandis in vitro regeneration |
Autor(es): | Rogalski, Marcelo Tambarussi, Evandro V. Galeano, Esteban Brondani, Gilvano Ebling Martin, Valentina de Fatima de Silva, Lucas Américo da Carrer, Helaine |
Abstract: | The aim of this study was to develop a shoot regeneration protocol for Tectona grandis by examining the effects of pretreatments, regeneration culture medium, adventitious rooting, and acclimatization. The best organogenic potential was achieved when hypocotyls and cotyledonary segments from seedlings germinated on a medium containing TDZ, were cultured for four weeks on MS medium supplemented with BAP/GA3. With TDZ pretreatment during seed germination, up to 70% of the cotyledonary segments (0.5 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1) and 60% of the hypocotyl explants (0.1 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1) resulted in shoot regeneration. Finally, 65% of the regenerated shoots rooted, of which 60% were successfully acclimatized ex vitro. Our protocol increases regeneration efficiency for teak and it can serve as a platform for genetic transformation to improve this important tree species. |
Palavras-chave: | Adventitious rooting Plant growth regulator Plant tissue culture Woody plant |
Editor: | Crop Breeding and Applied Biotechnology |
Tipo de Acesso: | Open Access |
URI: | http://dx.doi.org/10.1590/1984-70332017v17n2a19 http://www.locus.ufv.br/handle/123456789/18171 |
Data do documento: | 17-Mar-2017 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf | texto completo | 719,62 kB | Adobe PDF | Visualizar/Abrir |
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