Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/18171
Tipo: Artigo
Título: Efficient and new method for Tectona grandis in vitro regeneration
Autor(es): Rogalski, Marcelo
Tambarussi, Evandro V.
Galeano, Esteban
Brondani, Gilvano Ebling
Martin, Valentina de Fatima de
Silva, Lucas Américo da
Carrer, Helaine
Abstract: The aim of this study was to develop a shoot regeneration protocol for Tectona grandis by examining the effects of pretreatments, regeneration culture medium, adventitious rooting, and acclimatization. The best organogenic potential was achieved when hypocotyls and cotyledonary segments from seedlings germinated on a medium containing TDZ, were cultured for four weeks on MS medium supplemented with BAP/GA3. With TDZ pretreatment during seed germination, up to 70% of the cotyledonary segments (0.5 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1) and 60% of the hypocotyl explants (0.1 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1) resulted in shoot regeneration. Finally, 65% of the regenerated shoots rooted, of which 60% were successfully acclimatized ex vitro. Our protocol increases regeneration efficiency for teak and it can serve as a platform for genetic transformation to improve this important tree species.
Palavras-chave: Adventitious rooting
Plant growth regulator
Plant tissue culture
Woody plant
Editor: Crop Breeding and Applied Biotechnology
Tipo de Acesso: Open Access
URI: http://dx.doi.org/10.1590/1984-70332017v17n2a19
http://www.locus.ufv.br/handle/123456789/18171
Data do documento: 17-Mar-2017
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