Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/14628
Tipo: Artigo
Título: Differential expression of plg genes from Penicillium griseoroseum: plg1 a pectinolytic gene is expressed in sucrose and yeast extract
Autor(es): Bazzolli, D.M.S.
Ribon, A. de O.B.
Reis, K.C.P.
Queiroz, M.V. de
Araújo, E.F. de
Abstract: To study the regulation of the plg1 and plg2 genes of Penicillium griseoroseum, in order to identify the industrial potential of their products in alternative carbon sources that are cheaper and widely available in Brazil. RT-PCR and Northern blot were used to investigate if plg1 and plg2 expression is under influence of catabolic repression, ambient pH and cAMP. Results demonstrated that the genes were differentially regulated depending on the carbon sources in the culture medium and pH. Sucrose, a noninducing carbon source of the pectinolytic system, was able to promote plg1 transcription but only when yeast extract was added into the culture medium. The plg genes are differentially expressed. The plg1 gene is more attractive for industrial use due to its expression in alternative carbon sources like sucrose and yeast extract. In recent years, industries have been trying to replace the toxic conventional treatments employed in these processes by more eco-friendly enzyme treatment. Alternative carbon sources will be tested with the aim to reduce the costs associated to pectin lyase production in Brazil.
Palavras-chave: Enzyme production
Gene expression
Industrial applications
Pectinases
Pectin lyase
Penicillium
Sucrose and yeast extract
Editor: Journal of Applied Microbiology
Tipo de Acesso: Open Access
URI: http://dx.doi.org/10.1111/j.1365-2672.2008.03905.x
http://www.locus.ufv.br/handle/123456789/14628
Data do documento: 22-Abr-2008
Aparece nas coleções:Artigos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Bazzolli_et_al-2008-Journal_of_Applied_Microbiology.pdftexto completo247,82 kBAdobe PDFThumbnail
Visualizar/Abrir


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.