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https://locus.ufv.br//handle/123456789/13981
Tipo: | Artigo |
Título: | Interference of raw milk autochthonous microbiota on the performance of conventional methodologies for Listeria monocytogenes and Salmonella spp. detection |
Autor(es): | Nero, Luís Augusto Mattos, Marcos Rodriguesde Barros, Márcia de Aguiar Ferreira Beloti, Vanerli Franco, Bernadette Dora Gombossy de Melo |
Abstract: | Pathogen detection in foods by reliable methodologies is very important to guarantee microbiological safety. However, peculiar characteristics of certain foods, such as autochthonous microbiota, can directly influence pathogen development and detection. With the objective of verifying the performance of the official analytical methodologies for the isolation of Listeria monocytogenes and Salmonella in milk, different concentrations of these pathogens were inoculated in raw milk treatments with different levels of mesophilic aerobes, and then submitted to the traditional isolation procedures for the inoculated pathogens. Listeria monocytogenes was inoculated at the range of 0.2–5.2 log CFU/mL in treatments with 1.8–8.2 log CFU/mL. Salmonella Enteritidis was inoculated at 0.9–3.9 log CFU/mL in treatments with 3.0–8.2 log CFU/mL. The results indicated that recovery was not possible or was more difficult in the treatments with high counts of mesophilic aerobes and low levels of the pathogens, indicating interference of raw milk autochthonous microbiota. |
Palavras-chave: | Autochthonous microbiota Milk Pathogen detection Listeria monocytogenes Salmonella spp |
Editor: | Microbiological Research |
Tipo de Acesso: | Open Access |
URI: | https://doi.org/10.1016/j.micres.2007.04.003 http://www.locus.ufv.br/handle/123456789/13981 |
Data do documento: | 29-Set-2009 |
Aparece nas coleções: | Artigos |
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1-s2.0-S0944501307000651-main.pdf | texto completo | 193,51 kB | Adobe PDF | Visualizar/Abrir |
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