Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/13828
Tipo: Artigo
Título: A BOX-SCAR fragment for the identification of Actinobacillus pleuropneumoniae
Autor(es): Rossi, Ciro C.
Pereira, Monalessa F.
Langford, Paul R.
Bazzolli, Denise M. S.
Abstract: Bacterial respiratory diseases are responsible for considerable mortality, morbidity and economic losses in the swine industry. Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, is one of the most important disease agents, but its identification and surveillance can be impaired by the existence of many other related bacteria in normal swine microbiota. In this work, we have evaluated a BOX-A1R-based repetitive extragenic palindromic-PCR (BOX-PCR) sequence characterised amplified region (SCAR) marker for the specific identification of A. pleuropneumoniae and its use in a multiplex PCR to detect additionally Haemophilus parasuis and Pasteurella multocida, two other major respiratory pathogens of pigs that are members of the family Pasteurellaceae. PCRs based on the BOX-SCAR fragment developed were rapid, sensitive and differentiated A. pleuropneumoniae from all swine-related members of the Pasteurellaceae family tested. Single and multiplex BOX-SCAR fragment-based PCRs can be used to identify A. pleuropneumoniae from other bacterial swine pathogens and will be useful in surveillance and epidemiological studies.
Palavras-chave: BOX-PCR
Equence characterised amplified region
Multiplex PCR
Swine pleuropneumonia
Pasteurellaceae
Editor: FEMS Microbiology Letters
Tipo de Acesso: Open Access
URI: https://doi.org/10.1111/1574-6968.12363
http://www.locus.ufv.br/handle/123456789/13828
Data do documento: 6-Jan-2014
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