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https://locus.ufv.br//handle/123456789/12625
Tipo: | Artigo |
Título: | Recombinant Leishmania (Leishmania) infantum Ecto-Nucleoside Triphosphate Diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis |
Autor(es): | Souza, Ronny Francisco de Santos, Yaro Luciolo dos Vasconcellos, Raphael de Souza Borges-Pereira, Lucas Almeida, Márcia Rogéria de Fietto, Juliana Lopes Rangel Caldas, Ivo Santana Bahia, Maria Terezinha |
Abstract: | Canine visceral leishmaniasis is an important public health concern. In the epidemiological context of human visceral leishmaniasis, dogs are considered the main reservoir of Leishmania parasites; therefore, dogs must be epidemiologically monitored constantly in endemic areas. Furthermore, dog to human transmission has been correlated with emerging urbanization and increasing rates of leishmaniasis infection worldwide. Leishmania (Leishmania) infantum (L. chagasi) is the etiologic agent of visceral leish-maniasis in the New World. In this work, a new L. (L.) infantum (L. chagasi) recombinant antigen, named ATP diphosphohydrolase (rLic-NTPDase-2), intended for use in the immunodiagnosis of CVL was produced and validated. The extracellular domain of ATP diphosphohydrolase was cloned and expressed in the pET21b-Escherichia coli expression system. Indirect ELISA assays were used to detect the purified rLic-NTPDase-2 antigen using a standard canine sera library. This library contained CVL-positive samples, leishmaniasis-negative samples and samples from Trypanosoma cruzi-infected dogs. The results show a high sensitivity of 100% (95% CI = 92.60–100.0%) and a high specificity of 100% (95% CI = 86.77–100.0%),with a high degree of confidence (k = 1). These findings demonstrate the potential use of this recombinant protein in immune diagnosis of canine leishmaniasis and open the possibility of its application to other diagnostic approaches, such as immunochromatography fast lateral flow assays and human leishmaniasis diagnosis. |
Palavras-chave: | Canine leishmaniasis Immunodiagnosis Recombinant protein Ecto-Nucleoside Triphosphate Diphosphohydrolase |
Editor: | Acta Tropica |
Tipo de Acesso: | Open Access |
URI: | https://doi.org/10.1016/j.actatropica.2012.09.011 http://www.locus.ufv.br/handle/123456789/12625 |
Data do documento: | 26-Set-2012 |
Aparece nas coleções: | Artigos |
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1-s2.0-S0001706X12003166-main.pdf | texto completo | 527,87 kB | Adobe PDF | Visualizar/Abrir |
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