Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/12625
Tipo: Artigo
Título: Recombinant Leishmania (Leishmania) infantum Ecto-Nucleoside Triphosphate Diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis
Autor(es): Souza, Ronny Francisco de
Santos, Yaro Luciolo dos
Vasconcellos, Raphael de Souza
Borges-Pereira, Lucas
Almeida, Márcia Rogéria de
Fietto, Juliana Lopes Rangel
Caldas, Ivo Santana
Bahia, Maria Terezinha
Abstract: Canine visceral leishmaniasis is an important public health concern. In the epidemiological context of human visceral leishmaniasis, dogs are considered the main reservoir of Leishmania parasites; therefore, dogs must be epidemiologically monitored constantly in endemic areas. Furthermore, dog to human transmission has been correlated with emerging urbanization and increasing rates of leishmaniasis infection worldwide. Leishmania (Leishmania) infantum (L. chagasi) is the etiologic agent of visceral leish-maniasis in the New World. In this work, a new L. (L.) infantum (L. chagasi) recombinant antigen, named ATP diphosphohydrolase (rLic-NTPDase-2), intended for use in the immunodiagnosis of CVL was produced and validated. The extracellular domain of ATP diphosphohydrolase was cloned and expressed in the pET21b-Escherichia coli expression system. Indirect ELISA assays were used to detect the purified rLic-NTPDase-2 antigen using a standard canine sera library. This library contained CVL-positive samples, leishmaniasis-negative samples and samples from Trypanosoma cruzi-infected dogs. The results show a high sensitivity of 100% (95% CI = 92.60–100.0%) and a high specificity of 100% (95% CI = 86.77–100.0%),with a high degree of confidence (k = 1). These findings demonstrate the potential use of this recombinant protein in immune diagnosis of canine leishmaniasis and open the possibility of its application to other diagnostic approaches, such as immunochromatography fast lateral flow assays and human leishmaniasis diagnosis.
Palavras-chave: Canine leishmaniasis
Immunodiagnosis
Recombinant protein
Ecto-Nucleoside
Triphosphate
Diphosphohydrolase
Editor: Acta Tropica
Tipo de Acesso: Open Access
URI: https://doi.org/10.1016/j.actatropica.2012.09.011
http://www.locus.ufv.br/handle/123456789/12625
Data do documento: 26-Set-2012
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