Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/12544
Tipo: Artigo
Título: Computer aided identification of a Hevein-like antimicrobial peptide of bell pepper leaves for biotechnological use
Autor(es): Games, Patrícia Dias
Silva, Elói Quintas Gonçalves da
Barbosa, Meire de Oliveira
Almeida-Souza, Hebréia Oliveira
Fontes, Patrícia Pereira
Magalhães-Jr, Marcos Jorge de
Pereira, Paulo Roberto Gomes
Prates, Maura Vianna
Franco, Gloria Regina
Faria-Campos, Alessandra
Campos, Sérgio Vale Aguiar
Baracat-Pereira, Maria Cristina
Abstract: Antimicrobial peptides from plants present mechanisms of action that are different from those of conventional defense agents. They are under-explored but have a potential as commercial antimicrobials. Bell pepper leaves (‘Magali R’) are discarded after harvesting the fruit and are sources of bioactive peptides. This work reports the isolation by peptidomics tools, and the identification and partially characterization by computational tools of an antimicrobial peptide from bell pepper leaves, and evidences the usefulness of records and the in silico analysis for the study of plant peptides aiming biotechnological uses. Aqueous extracts from leaves were enriched in peptide by salt fractionation and ultrafiltration. An antimicrobial peptide was isolated by tandem chromatographic procedures. Mass spectrometry, automated peptide sequencing and bioinformatics tools were used alternately for identification and partial characterization of the Hevein-like peptide, named HEV-CANN. The computational tools that assisted to the identification of the peptide included BlastP, PSI-Blast, ClustalOmega, PeptideCutter, and ProtParam; conventional protein databases (DB) as Mascot, Protein-DB, GenBank-DB, RefSeq, Swiss-Prot, and UniProtKB; specific for peptides DB as Amper, APD2, CAMP, LAMPs, and PhytAMP; other tools included in ExPASy for Proteomics; The Bioactive Peptide Databases, and The Pepper Genome Database. The HEV-CANN sequence presented 40 amino acid residues, 4258.8 Da, theoretical pI-value of 8.78, and four disulfide bonds. It was stable, and it has inhibited the growth of phytopathogenic bacteria and a fungus. HEV-CANN presented a chitin-binding domain in their sequence. There was a high identity and a positive alignment of HEV-CANN sequence in various databases, but there was not a complete identity, suggesting that HEV-CANN may be produced by ribosomal synthesis, which is in accordance with its constitutive nature. Computational tools for proteomics and databases are not adjusted for short sequences, which hampered HEV-CANN identification. The adjustment of statistical tests in large databases for proteins is an alternative to promote the significant identification of peptides. The development of specific DB for plant antimicrobial peptides, with information about peptide sequences, functional genomic data, structural motifs and domains of molecules, functional domains, and peptide-biomolecule interactions are valuable and necessary
Palavras-chave: Hevein-like
Antimicrobial peptide
Bell pepper
Plant defense
Peptidomics
Computational tools
Bioinformatics
Biotechnology
Editor: BioMed Central Genomics
Tipo de Acesso: Open Access
URI: http://dx.doi.org/10.1186/s12864-016-3332-8
http://www.locus.ufv.br/handle/123456789/12544
Data do documento: 15-Dez-2016
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