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https://locus.ufv.br//handle/123456789/12455
Tipo: | Artigo |
Título: | The influence of pretreatment methods on saccharification of sugarcane bagasse by an enzyme extract from Chrysoporthe cubensis and commercial cocktails: A comparative study |
Autor(es): | Maitan-Alfenas, Gabriela Piccolo Visser, Evan Michael Alfenas, Rafael Ferreira Nogueira, Bráulio Ris G. Campos, Guilherme Galvão de Milagres, Adriane Ferreira Vries, Ronald P. de Guimarães, Valéria Monteze |
Abstract: | Biomass enzymatic hydrolysis depends on the pretreatment methods employed, the composition of initial feedstock and the enzyme cocktail used to release sugars for subsequent fermentation into ethanol. In this study, sugarcane bagasse was pretreated with 1% H2SO4 and 1% NaOH and the biomass saccharification was performed with 8% solids loading using 10 FPase units/g of bagasse of the enzymatic extract from Chrysoporthe cubensis and three commercial cocktails for a comparative study. Overall, the best glucose and xylose release was obtained from alkaline pretreated sugarcane bagasse. The C. cubensis extract promoted higher release of glucose (5.32 g/L) and xylose (9.00 g/L) than the commercial mixtures. Moreover, the C. cubensis extract presented high specific enzyme activities when compared to commercial cocktails mainly concerning to endoglucanase (331.84 U/mg of protein), β-glucosidase (29.48 U/mg of protein), β-xylosidase (2.95 U/mg of protein), pectinase (127.46 U/mg of protein) and laccase (2.49 U/mg of protein). |
Palavras-chave: | Chrysoporthe cubensis Enzyme mixtures Biomass pretreatment Saccharification |
Editor: | Bioresource Technology |
Tipo de Acesso: | Open Access |
URI: | https://doi.org/10.1016/j.biortech.2015.05.109 http://www.locus.ufv.br/handle/123456789/12455 |
Data do documento: | 14-Jun-2015 |
Aparece nas coleções: | Artigos |
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1-s2.0-S0960852415008330-main.pdf | texto completo | 572,15 kB | Adobe PDF | Visualizar/Abrir |
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